Journal: Frontiers in Plant Science

Article Title: Hydroxyanthracene derivates citotoxicity: A differential evaluation between single molecule and whole plant extract

doi: 10.3389/fpls.2023.1166075

Figure Lengend Snippet: Cell viability of Caco-2 cells at different concentrations of different plant extracts and isolated compounds by alamarBlue viability assay. (A) Caco-2 cells were incubated for 48 h with different concentrations (1–20 ppm) of single molecule (rhein) or plant extract ( Cassia angustifolia ) at the same concentrations (1–20 ppm). (B) Caco-2 cells were incubated for 48 h with different concentrations (1–20 ppm) of single molecule (rhein) or plant extract ( Rheum palmatum ) at the same concentrations (1–20 ppm). (C) Caco-2 cells were incubated for 48 h with different concentrations (1–20 ppm) of single molecules (emodin and aloe-emodin) or plant extract ( Rhamnus purshiana ) at the same concentrations (1–20 ppm). (D) Caco-2 cells were incubated for 48 h with different concentrations (1–20 ppm) of single molecule (emodin) or plant extract ( Rhamnus frangula ) at the same concentrations (1–20 ppm). *P ≤ 0.05; **P ≤ 0.01.

Article Snippet: The Caco-2 treatment was performed for 48 h. Then, the oxidized alamarBlue reagent (Merck Group) was added in each well at a final concentration of 0.15 mg/ml, and cells were maintained for 3 h in the standard culture conditions.

Techniques: Isolation, Viability Assay, Incubation, Plant Extract

Journal: Pathogens

Article Title: Ursolic Acid Derivatives as Potential Agents Against Acanthamoeba Spp.

doi: 10.3390/pathogens8030130

Figure Lengend Snippet: The 50% inhibitory concentrations (IC 50s ) , the cytotoxic concentrations 50 (CC 50 ) in µM and the selectivity index (SI) values for the compounds used in this study against Acanthamoeba spp. and murine macrophages measured using the Alamar blue assay. Mean concentration ± SD.

Article Snippet: Finally, the Alamar Blue assay reagent (Bioresource, Europe, Nivelles, Belgium) was added into each well at an amount equal to 10% of the medium volume.

Techniques: Alamar Blue Assay, Concentration Assay